DLEU1 promotes cell survival by preventing DYNLL1 degradation in esophageal squamous cell carcinoma

• DOI: 10.1186/s12967-022-03449-w
• Publication Date: 2022-05-26T11:04:06Z
• Authors: Qihang Li; Zhiyu Zhang; HongChao Jiang; Jun Hou; Yuhang Chai; Hongxing Nan; Feng Li; Lianghai Wang
• Keywords: Viability assay

Abstract Background Emerging evidence has highlighted the critical roles of long noncoding RNAs (lncRNAs) in tumor development and progression. However, biological functions underlying mechanisms DLEU1 esophageal squamous cell carcinoma (ESCC) remain unclear. Methods LncRNA expression ESCC tissues was explored using lncRNA microarray datasets. The functional were demonstrated by a series vitro vivo experiments. RNA pull-down immunoprecipitation assays performed to demonstrate potential DLEU1. Results In screen for differentially expressed lncRNAs ESCC, we determined that one most overexpressed upregulated associated with worse prognosis. Functional showed promoted growth inhibiting apoptosis. Mechanistically, could bind stabilize DYNLL1 interfering RNF114-mediated ubiquitination proteasomal degradation. DLEU1/DYNLL1 axis subsequently antiapoptotic BCL2 survival. Furthermore, upregulation at least partly facilitated promoter hypomethylation. Notably, targeting sensitized cells cisplatin-induced death. Conclusions Our findings suggest DLEU1-mediated stabilization is survival may be promising therapeutic target ESCC.