Lymphatic metastasis-associated circRNA‒miRNA‒mRNA network for exploring the pathogenesis and therapeutic target of triple negative breast cancer based on whole-transcriptome sequencing analysis: an experimental verification study

• DOI: 10.1186/s12967-022-03728-6
• Publication Date: 2022-11-05T06:02:31Z
• Authors: Jiayue Luo; Dong Cao; Chuwen Hu; Zhen Liang; Yuanping Zhang; Jianguo Lai
• Keywords: Competing Endogenous RNA; Triple-negative breast cancer

The metastatic mechanisms of axillary lymph nodes (ALNs) in triple-negative breast cancer (TNBC) remain unclear. We aimed to identify the potential circRNA regulatory network ALN metastasis.We performed whole transcriptome sequencing (WTS) determine expression profiles RNAs and screen out differentially expressed messenger (DEMs), microRNAs (DEMis), circRNAs (DECs) between ALN-positive ALN-negative TNBC patients. Functional enrichment analysis Kaplan-Meier survival were utilized unearth DEMs. A competing endogenous RNA (ceRNA) was constructed using computational biology. levels DECs cell lines confirmed by real-time polymerase chain reaction (RT‒PCR).Following WTS differential analysis, 739 DEMs, 110 DEMis, 206 identified indicated that DEMs mainly functioned carcinogenesis tumor progression-related pathways. ceRNA networks containing eight circRNAs, six miRNAs, eighteen mRNAs developed. In network, two (RAB3D EDARADD) significantly associated with better overall one mRNA (GSR) predicted favorable recurrence-free patients chosen for further analysis. Then, a survival-related (hsa_circ_0061260 hsa_circ_0060876), DEMis (hsa-miR-5000-3p hsa-miR-4792), three (GSR, RAB3D, identified. candidate validated PCR.Our research provides novel insights into molecular mechanism metastasis therapeutic targets TNBC.