A circular network of purine metabolism as coregulators of dilated cardiomyopathy

• DOI: 10.1186/s12967-022-03739-3
• Publication Date: 2022-11-18T12:03:38Z
• Authors: Ge Wang; Rongjun Zou; Libao Liu; Zongtao Wang; Zengxiao Zou; Songtao Tan; Wenliu Xu; Xiaoping Fan
• Keywords: Purine metabolism; Pyrimidine metabolism

Abstract Background The crosstalk of purine biosynthesis and metabolism exists to balance the cell energy production, proliferation, survival cytoplasmic environment stability, but disorganized mechanics with respect developing heart failure (HF) is currently unknown. Methods We conducted a multi-omics wide analysis, including microarray-based transcriptomes, full spectrum metabolomics chronic HF. Based on expression profiling by array, we applied bioinformatics platform quantifiable metabolic pathway changes based gene set enrichment analysis (GSEA), variation (GSVA), Shapley Additive Explanations (SHAP), Xtreme Gradient Boosting (XGBoost) algorithms comprehensively analyze dynamic pathways circular network in HF development. Additionally, left ventricular tissue from patients undergoing myocardial biopsy transplantation were collected perform protein mass spectrometry. Results Systematic showed reprogramming was significantly detected dilated cardiomyopathy. In addition, this result also demonstrated metabolomic And differentially expressed metabolites showing guanine, urea, xanthine detected. Hub markers, includes IMPDH1 , ENTPD2 AK7 AK2 CANT1 identified XGBoost, SHAP model PPI network. Conclusion reactions involved may involving DCM reprogramming, as coregulators development HF, which identify potential therapeutic targets. markers shown an important role.