The HIFIA/LINC02913/IGF1R axis promotes the cell function of adipose-derived mesenchymal stem cells under hypoxia via activating the PI3K/AKT pathway

• DOI: 10.1186/s12967-023-04581-x
• Publication Date: 2023-10-17T16:01:44Z
• Authors: Xiang Xiong; Liqin Yuan; Kai Yang; Xiancheng Wang
• Keywords: Hypoxia

Promoting angiogenesis is crucial for tissue repair. Adipose-derived mesenchymal stem cells (ADSCs) are endowed with the ability of paracrine secretion various angiogenic cytokines and differentiation potential into endothelium-like to directly participate in angiogenesis. ADSCs key seed promoting regenerative medicine engineering. This study aimed explore role mechanism C9orf106 (LINC02913) ADSCs.The microarray dataset GSE12884 was analyzed identify differentially expressed lncRNAs under normoxia hypoxia. The expression genes detected using qRT-PCR, western blot assay (western blot), immunofluorescence (IF) staining. adipogenic tube formation oil red O staining assay, respectively. regulatory relationship between hypoxia-inducible factor-1alpha (HIF1A) LINC02913 verified chromatin immunoprecipitation (ChIP) dual-luciferase reporter gene assay. A skin wound healing nude mice model established. Hematoxylin eosin (H&E) applied detect pathological damage. Immunohistochemistry (IHC) used determine level CD31 tissues.LINC02913 decreased hypoxia; overexpression inhibited proliferation, ability, endothelial ADSCs. ChIP results showed that HIF1A could bind promoter region inhibit its transcription. Through RNAact prediction analysis correlation expression, it found IGF1R may interact LINCO02913. HIF1A/LINC02913/IGF1R axis activate PI3K/AKT pathway promote biological function Hypoxia-ADSCs significantly promoted vascularization wounded skin. effect LINC02913/IGF1R on hypoxia-ADSCs treated were verified.The hypoxia via activating pathway.