Oxytocin inhibits lipopolysaccharide-induced inflammation in microglial cells and attenuates microglial activation in lipopolysaccharide-treated mice

• DOI: 10.1186/s12974-016-0541-7
• Publication Date: 2016-04-14T06:43:39Z
• Authors: Lin Yuan; Song Liu; Xuemei Bai; Yan Gao; Guangheng Liu; Xueer Wang; Dexiang Liu; Tong Li; Aijun Hao; Zhen Wang
• Keywords: Inflammation; Lipopolysaccharides; 0301 basic medicine; Mice, Inbred BALB C; Reverse Transcriptase Polymerase Chain Reaction; Research; Immunology; Blotting, Western; Anti-Inflammatory Agents; Brain; Fluorescent Antibody Technique; Oxytocin; 3. Good health; Cellular and Molecular Neuroscience; Mice; 03 medical and health sciences; Neurology; Animals; Microglia

Overactivated microglia is involved in various kinds of neurodegenerative diseases. Suppression microglial overactivation has emerged as a novel strategy for treatment neuroinflammation-based neurodegeneration. In the current study, anti-inflammatory effects oxytocin (OT), which highly conserved nonapeptide with hormone and neurotransmitter properties, were investigated vitro vivo. BV-2 cells primary pre-treated OT (0.1, 1, 10 μM) 2 h followed by LPS (500 ng/ml); activation pro-inflammatory mediators measured Western blot, RT-PCR, immunofluorescence. The MAPK NF-κB pathway proteins assessed blot. intracellular calcium concentration ([Ca2+]i) was determined using Fluo2-/AM assay. Intranasal application BALB/C mice (adult male) injected intraperitoneally (5 mg/kg). effect on LPS-induced immunofluorescence Using cell line microglia, we found that pre-treatment significantly inhibited reduced subsequent release factors. addition, phosphorylation ERK p38 but not JNK microglia. remarkably elevation [Ca2+]i LPS-stimulated cells. Furthermore, systemic LPS-treated acute inflammation murine brain model used to study suppressive against neuroinflammation We showed marked attenuation factor levels. Taken together, present demonstrated possesses anti-neuroinflammatory activity might serve potential therapeutic agent treating neuroinflammatory