Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia

• DOI: 10.1186/s12974-018-1081-0
• Publication Date: 2018-02-09T07:27:47Z
• Authors: Xiangrong Liu; Shaohong Wen; Feng Yan; Kuan Liu; Liqiang Liu; Lei Wang; Shangfeng Zhao; Xunming Ji
• Keywords: Inflammation; Male; 0301 basic medicine; Dose-Response Relationship, Drug; Research; Cell Polarity; Neuron; Neuroprotection; Brain Ischemia; Rats; 3. Good health; Stroke; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Glucosides; Phenols; Polarization; Salidroside; Animals; Neurology. Diseases of the nervous system; Microglia; RC346-429; Cells, Cultured

Following stroke, microglia can be driven to the "classically activated" pro-inflammatory (M1) phenotype and "alternatively anti-inflammatory (M2) phenotype. Salidroside (SLDS) is known inhibit inflammation possess protective effects in neurological diseases, but date, exact mechanisms involved these processes after stroke have yet elucidated. The purpose of this study was determine SLDS on neuroprotection microglial polarization stroke.Male adult C57/BL6 mice were subjected focal transient cerebral ischemia followed by intravenous injection. optimal dose determined evaluation infarct volume functions. RT-PCR immunostaining performed assess polarization. A transwell system a direct-contact coculture used elucidate SLDS-induced oligodendrocyte differentiation neuronal survival.SLDS significantly reduced infarction improved function ischemia. treatment expression M1 microglia/macrophage markers increased M2 induced primary from Furthermore, enhanced phagocytosis suppressed microglial-derived inflammatory cytokine release. Cocultures oligodendrocytes SLDS-treated resulted differentiation. Moreover, protected neurons against oxygen glucose deprivation promoting polarization.These data demonstrate that protects modulating An understanding SLDS-mediated may lead new therapeutic opportunities stroke.