MxB impedes the NUP358-mediated HIV-1 pre-integration complex nuclear import and viral replication cooperatively with CPSF6

• DOI: 10.1186/s12977-020-00524-2
• Publication Date: 2020-06-29T12:04:05Z
• Authors: Linlin Xie; Lang Chen; Chaojie Zhong; Ting Yu; Zhao Ju; Meirong Wang; Hairong Xiong; Yan Zeng; Jianhua Wang; Haitao Hu; Wei Hou; Yong Feng
• Keywords: Replication; Nuclear export signal

Abstract Background The human myxovirus resistance 2 (Mx2/MxB) protein was originally found to regulate cytoplasmic-nuclear transport but recently reported restrict HIV-1 replication by binding capsid (CA), preventing uncoating, the nuclear import of pre-integration complex (PIC) and viral DNA integration. This work explores mechanisms MxB-mediated inhibition. Results We demonstrated that MxB represses NUP358-mediated PIC replication. Moreover, MxB’s effects on depend critically cofactor cleavage polyadenylation specificity factor subunit 6 (CPSF6). binds nucleoporin NUP358, blocks NUP358-CA interaction, thereby impeding with CPSF6 PIC. More intriguingly, CPSF6’s role in depends MxB, being a facilitator its own, becoming an inhibitor when is present. Conclusions Our establishes impedes cooperatively CPSF6.