A novel Betaretrovirus discovered in cattle with neurological disease and encephalitis

• DOI: 10.1186/s12977-021-00585-x
• Publication Date: 2021-12-20T10:03:12Z
• Authors: Melanie M. Hierweger; Michel C. Koch; Ronja V. Kauer; Zoltán Bagó; Anna Oevermann; Giuseppe Bertoni; Torsten Seuberlich
• Keywords: 0301 basic medicine; 610 Medicine & health; Betaretrovirus; 03 medical and health sciences; Animals; Virus discovery; Phylogeny; Retrospective Studies; 0303 health sciences; High-throughput sequencing; 630 Agriculture; Research; 500 Science; RC581-607; 3. Good health; Bovine retrovirus Cattle High-throughput sequencing Neurological disease Non-suppurative encephalitis Virus discovery; Non-suppurative encephalitis; Bovine retrovirus; Viruses; 570 Life sciences; biology; 590 Animals (Zoology); Encephalitis; Cattle; Female; Immunologic diseases. Allergy; Neurological disease

Abstract Background The majority of emerging infectious diseases in humans are of animal origin, and many of them are caused by neuropathogenic viruses. Many cases of neurological disease and encephalitis in livestock remain etiologically unresolved, posing a constant threat to animal and human health. Thus, continuous extension of our knowledge of the repertoire of viruses prone to infect the central nervous system (CNS) is vital for pathogen monitoring and the early detection of emerging viruses. Using high-throughput sequencing (HTS) and bioinformatics, we discovered a new retrovirus, bovine retrovirus CH15 (BoRV CH15), in the CNS of a cow with non-suppurative encephalitis. Phylogenetic analysis revealed the affiliation of BoRV CH15 to the genus Betaretrovirus.

Results BoRV CH15 genomes were identified prospectively and retrospectively by PCR, RT-PCR, and HTS, with targeting of viral RNA and proviral DNA, in six additional diseased cows investigated over a period of > 20 years and of different geographical origins. The virus was not found in brain samples from healthy slaughtered control animals (n = 130). We determined the full-length proviral genomes from six of the seven investigated animals and, using in situ hybridization, identified viral RNA in the cytoplasm of cells morphologically compatible with neurons in diseased brains.

Conclusions Further screening of brain samples, virus isolation, and infection studies are needed to estimate the significance of these findings and the causative association of BoRV CH15 with neurological disease and encephalitis in cattle. However, with the full-length proviral sequences of BoRV CH15 genomes, we provide the basis for a molecular clone and further in vitro investigation.

Graphical Abstract