Repeated oral administration of low doses of silver in mice: tissue distribution and effects on central nervous system

• DOI: 10.1186/s12989-021-00418-x
• Publication Date: 2021-06-16T09:03:03Z
• Authors: Camilla Recordati; Marcella De Maglie; Claudia Cella; Simona Argentiere; Saverio Paltrinieri; Silvia Bianchessi; Marco Losa; Fabio Fiordaliso; Alessandro Corbelli; Gianpaolo Milite; Federica Aureli; Marilena D’Amato; Andrea Raggi; Francesco Cubadda; Sabina Soldati; Cristina Lenardi; Eugenio Scanziani
• Keywords: Silver nanoparticle; Silver acetate; Silver stain

Abstract Background Widespread use of silver in its different forms raises concerns about potential adverse effects after ingestion, the main exposure route for humans. The aim this study was to investigate CD-1 (ICR) male mice tissue distribution and vivo 4-week oral 0.25 1 mg Ag/kg bw 10 nm citrate coated nanoparticles (AgNPs) acetate (AgAc) at end treatment (EoT) 4 weeks recovery. Results There were no treatment-related clinical signs mortality, significant on body organ weights EoT Treatment-related changes hematology chemistry found recovery, most relevant being a dose-dependent lymphopenia increased triglycerides AgNP-treated mice, levels urea all treated groups, associated with decreased albumin only AgAc-treated mice. At highest concentration determined by Triple Quadrupole ICP-MS analysis brain, followed testis, liver, spleen; much lower concentrations present small intestine kidney. Tissue slightly higher AgAc than AgNPs dose dependent AgNPs. After recovery still brain highlighting slow elimination. No histopathological absence staining autometallography observed organs GFAP (astrocytes) immunoreactivity significantly hippocampus manner Iba1 (microglial cells) cortex mg/kg reduction groups. TEM revealed splitting basement membrane capillaries swelling astrocytic perivascular end-feet AgNP- EoT. Conclusions Our accumulation clearance administration low doses glial cells ultrastructural alterations Blood-Brain Barrier.