Assessment of a panel of tumor markers for the differential diagnosis of benign and malignant effusions by well-based reverse phase protein array

• DOI: 10.1186/s13000-015-0290-4
• Publication Date: 2015-05-28T16:23:00Z
• Authors: Till Braunschweig; Joon-Yong Chung; Chel Hun Choi; Hanbyoul Cho; Qing-Rong Chen; Ran Xie; Candice Perry; Javed Khan; Stephen M Hewitt
• Keywords: Malignant Transformation

The differential diagnosis of benign and malignant effusion is often hampered by low cell content or insufficiently preserved tumor cells. In this study, we evaluated the combined diagnostic value six markers measured well-based reverse-phase protein array (RPPA) for effusion. A total 114 patients (46 with effusions, 32 probable 36 effusions) were enrolled. Expressional levels MUC1, EMA, Pan-CK, HSP90, TGF-β CA125 determined RPPA. Median relative expression Pan-CK EMA significantly higher in than those (p < 0.001, p = 0.003, respectively), whereas level effusions lower that other groups 0.005). For predicting malignancy, presented best areas under curve 0.728 followed MUC1 0.701. sensitivity 52.0% 48.0% not better cytology. However, sensitivity, negative predictive value, accuracy marker panel cytology 14.7%, 7.5%, 6.1%, respectively. Tumor RPPA showed values between effusions. Further large scale studies need to be performed evaluate utility markers. virtual slide(s) article can found here: http://www.diagnosticpathology.diagnomx.eu/vs/1433424467160224