Unique microRNAs in lung adenocarcinoma groups according to major TKI sensitive EGFR mutation status

• DOI: 10.1186/s13000-015-0339-4
• Publication Date: 2015-07-13T13:45:28Z
• Authors: Min Gyoung Pak; Chang-Hun Lee; Woo-Jeong Lee; Dong-Hoon Shin; Mee-Sook Roh
• Keywords: Male; 0301 basic medicine; Histology; Lung Neoplasms; DNA Mutational Analysis; Adenocarcinoma of Lung; Kaplan-Meier Estimate; Adenocarcinoma; Pathology and Forensic Medicine; 03 medical and health sciences; Biomarkers, Tumor; Humans; Precision Medicine; Aged; Neoplasm Staging; Oligonucleotide Array Sequence Analysis; Research; Gene Expression Profiling; Exons; Middle Aged; 3. Good health; ErbB Receptors; Gene Expression Regulation, Neoplastic; MicroRNAs; Mutation; Female; miRNA; miR-34c; miR-183; miR-210; NSCLC; Epidermal growth factorreceptor

Lung cancer is the leading cause of mortality, despite development therapeutic strategies. Altered expression microRNAs(miRNAs) in human malignancies have been well recognized as diagnostic and prognostic indicators, including lung cancer. This study aims to delineate clinicopathologic significance three unique miRNAs adenocarcinoma according major sensitive EGFR mutation status. One-hundred formalin-fixed paraffin-embedded (FFPE) tissues were collected from patients who underwent surgery epidermal growth factor receptor (EGFR) study. The samples divided into groups which include exons 19 21 wild type. Some representative cases each group profiled using commercial miRNA microarray plates. Three significant selected they validated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), collective FFPE samples. We identified microRNAs (miR-34c, miR-183, miR-210) showed significantly altered all Compared normal control tissue, down-regulation miR-34c up-regulation miR-183 miR-210 caner (p < 0.05 for each). qRT-PCR. Expression levels miR-34c, different between = 0.034, <0.000, 0.036, respectively). Moreover, level was higher than type 0.028). Higher positively related poor tumor differentiation. Increased associated with lymphovascular invasion 0.037). Aberrant independently T stage 0.019), TNM 0.007). However, there noted a limited statistical significance. In exon group, high overall survival low one univariate Kaplan-Meier method. 0.035). Here, we show that may act potential suppressor gene oncogenic role pulmonary adenocarcinoma. also suggests group. Consequently, further studies biology lead biomarkers