MiR-186 suppresses the growth and metastasis of bladder cancer by targeting NSBP1

• DOI: 10.1186/s13000-015-0372-3
• Publication Date: 2015-08-19T13:35:33Z
• Authors: Kun Yao; Leye He; Yu Gan; Qing Zeng; Yingbo Dai; Jing Tan
• Keywords: 0303 health sciences; Histology; Epithelial-Mesenchymal Transition; Research; Blotting, Western; Real-Time Polymerase Chain Reaction; Pathology and Forensic Medicine; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Urinary Bladder Neoplasms; Trans-Activators; HMGN Proteins; Humans; Neoplasm Invasiveness

Increasing evidence has shown that microRNAs function as oncogenes or tumor suppressors in human malignancies, but the roles of miR-186 bladder cancer (BC) is still unclear.First, quantitative real-time PCR (qRT-PCR) was performed to detect expression tissues and cell lines. Then, Bioinformatics analysis, combined with luciferase reporter assay demonstrated target gene miR-186. Finally, regulation proliferation invasion were further investigated.Here, our study showed down-regulated Luciferase targets NSBP1 3'-untranslated region (UTR) directly suppresses (HMGN5) cells. siRNA- miR-186-mediated knock-down experiments revealed through suppression expression. Expression analysis a set epithelial-mesenchymal transition (EMT) markers involves suppressed EMT which reducing mesenchymal (vimentin N-cadherin) inducing epithelial marker (E-cadherin).Our data first time identified upstream regulator also suggest miR-186-suppressed novel therapeutic approach for cancer.