Healing of the Achilles tendon in rabbits—evaluation by magnetic resonance imaging and histopathology

• DOI: 10.1186/s13018-014-0132-1
• Publication Date: 2014-12-11T09:34:18Z
• Authors: Wilson Campos Tav...; Ubiratam Brum de ...; Eduardo Paulino; Leonardo de Souza...; Ana Paula Madureira; Maria Angélica Ba...; Daniel Victor Mor...; Adriana Maria Kak...; Vivian Resende
• Keywords: Tendon sheath; Contrast Enhancement; Histopathology; Histology

Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) could provide valuable findings for tendon regeneration. A non-invasive image method that can effectively evaluate the quality of scar tissue has not yet been employed. Thirteen New Zealand rabbits were divided into two groups: group 1—non-treated control (n = 4); 2—surgical intervention 9). The central portion Achilles was resected, and after 30 days, DCE-MRI performed. Contrast enhancement methods applied using region interest (ROI) technique. In medium third tendon, intra-substantial signal intensity presence hyper-intense intra-tendon focus points heterogeneity evaluated. Antero-posterior transversal diameters measured. removed dissected free from other tissues. Sections part stained histological analysis. difference between contrast curves surgical groups (p < 0.0001) observed. had an intense in sequences, enlargement diameter alteration, with hyper-signal widening sheath, which presented irregular contours enhancement. On histology, diffuse sheath wedge-shaped areas scarring rich disordered collagen fibres. These related to alteration intensity, DCE-MRI. MRI perfusion be a useful technique evaluating fibrous tendons.