MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

• DOI: 10.1186/s13019-021-01698-2
• Publication Date: 2021-11-12T12:02:55Z
• Authors: Jiangwei Hu; Xinqin Xiang; Wei Guan; Weihua Lou; Junming He; Jian Chen; Yin Fu; Guoliang Lou
• Keywords: Gentamicin protection assay

Abstract Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression LUSC was measured by qRT-PCR. Its impacts on tumor-related behaviors were investigated CCK8 assay, scratch healing flow cytometry and Transwell invasion methods. In addition, interaction between CDCA4 also elucidated through rescue experiment, western blot, dual-luciferase, bioinformatics analysis. Results Low level of confirmed tissue cells. Overexpressed markedly inhibited cancer progression. restrained expression. Rescue assay showed that overexpressing eliminated effect overexpressed CDCA4. Conclusion By targeting CDCA4, development LUSC.