Huiyang Shengji decoction promotes wound healing in diabetic mice by activating the EGFR/PI3K/ATK pathway

• DOI: 10.1186/s13020-021-00497-0
• Publication Date: 2021-11-02T17:07:00Z
• Authors: Qingwu Liu; Jinchao Zhang; Xuyang Han; Jia Chen; Yating Zhai; Yan Lin; Huike Ma; Fang Feng; Xiujuan He; Ping Li
• Keywords: Calycosin; Decoction; Chronic wound; Kampo

Common chronic wounds include diabetic ulcers, venous and pressure ulcers. The traditional Chinese medicine Huiyang Shengji decoction (HYSJD) has been shown to promote the healing of wounds, however, its pharmacological mechanism is still unclear.This study aimed determine HYSJD in promoting skin ulcers.Ultra-performance liquid chromatography was combined with tandem mass spectrometry (UPLC-MS/MS) analyze main components absorbed mouse serum at 30 min after oral administration HYSJD. db/db models for ulcers were constructed by full-thickness resection. Wound tissues day 7 post wound formation used perform microarray analysis growth factors chemokine expression. GO KEGG enrichment performed on differentially expressed proteins. ELISA assays measure differential cytokines Western blot expression levels related pathway proteins wounds.UPLC-MS/MS showed that chemical flavonoids, terpenes, alkaloids, phenylpropanoids, carbohydrates. At HYSJD, five detected serum, these included formononetin, calycosin, hypaconitine, calycosin-7-glucoside, sinapic acid. found increase rate mice days 3, 7, 14 formation, proliferation epidermal cells. Two between different groups, i.e., IGF-1 EGFR, further validated. Serum EGFR treatment group significantly increased. suggested PI3K/AKT involved cells mice. Experimental verification activated signaling skin.HYSJD promotes increasing activating pathway. Our provides an experimental basis