Evaluation of passive immunity transfer in Danish dairy calves measured by enzyme-linked immunosorbent assay and Brix refractometer

• DOI: 10.1186/s13028-025-00801-0
• Publication Date: 2025-03-30T02:07:49Z
• Authors: Anne Marie Michelsen; Nina Dam Otten; Mogens Vestergaard; Liza Rosenbaum Ni...; Bodil Højlund Nie...; Henrik Laessøe Ma...; Nynne Capion; Mette Bisgaard Pe...
• Keywords: Refractometer

Calves are born agammaglobulinemic and depend on transfer of passive immunity from colostrum. Failure (FTPI) adequate (ATPI) defined as serum immunoglobulin G (IgG) levels < 10 ≥ mg/mL, respectively. The objective this study was to evaluate the level passively transferred in Danish dairy calves measured with Enzyme-linked immunosorbent assay (ELISA) Brix refractometer. Blood samples 834 (430 bull 404 heifer calves) aged two nine days were included study. Serum IgG concentration determined by ELISA percentage (%Brix) a digital Median 17.05 mg/mL mean %Brix 8.5%. A total 592 (71.0%) 242 (29.0%) had For 541 (64.9%) 293 (35.1%) 8.1 8.1%, concentrations measurements highly correlated (r = 0.88). seems low does not meet new recommendations. However, applying cut-offs based radial immunodiffusion derived straightforward determination cut-off values for FTPI recommended.