Networked neural spheroid by neuro-bundle mimicking nervous system created by topology effect

• DOI: 10.1186/s13041-015-0109-y
• Publication Date: 2015-03-21T21:35:59Z
• Authors: Gi Seok Jeong; Joon Young Chang; Ji Soo Park; Seung-A Lee; DoYeun Park; Junsung Woo; Heeyoung An; C Justin Lee; Sang-Hoon Lee
• Keywords: Psychopharmacology; Spheroid

In most animals, the nervous system consists of central (CNS) and peripheral (PNS), latter which connects CNS to all parts body. Damage and/or malfunction causes serious pathologies, including neurodegenerative disorders, spinal cord injury, Alzheimer's disease. Thus, not surprising, considerable research effort, both in vivo vitro, has been devoted studying signal transmission through it. However, conventional vitro cell culture systems do enable control over diverse aspects neural microenvironment. Moreover, formation certain growth patterns remains a challenge. this study, we developed deep hemispherical, microchannel-networked, concave array applied it generate three-dimensional nerve-like bundles. The hemicylindrical channel network was easily fabricated by exploiting meniscus induced surface tension liquid poly(dimethylsiloxane) (PDMS) prepolymer. Neurospheroids spontaneously aggregated each microwell were networked neighboring spheroids channel. Notably, two types satellite also formed hemispherical microchannels self-aggregation acted as an anchoring point enhance networks with spheroids. During neural-network formation, progenitor cells successfully differentiated into glial neuronal cells. These secreted laminin, forming extracellular matrix around host Electrical stimuli transmitted between neurospheroids resulting bundle, detected imaging Ca(2+) signals responding