Long non‐coding RNA NORAD promotes the prostate cancer cell extracellular vesicle release via microRNA-541-3p-regulated PKM2 to induce bone metastasis of prostate cancer

• DOI: 10.1186/s13046-021-01891-0
• Publication Date: 2021-03-16T04:53:32Z
• Authors: Chuan-yi Hu; Juan Chen; Xin-hua Qin; Pan You; Jie Ma; Jing Zhang; He Zhang; Ji-dong Xu
• Keywords: Exosome

Abstract Background Bone metastasis is the leading cause of mortality and reduced quality life in patients with metastatic prostate cancer (PCa). Long non-coding RNA activated by DNA damage (NORAD) has been observed to have an abnormal expression various cancers. This article aimed explore molecular mechanism underlying regulatory role NORAD bone PCa. Methods clinical PCa tissues cell lines was detected application qRT-PCR. Cancer cells were then transfected plasmids expressing NORAD, after which Transwell assay CCK-8 carried out detect proliferation, migration, downstream target molecules screened through bioinformatics analysis, followed further verification using dual luciferase assay. Extracellular vesicles (EVs) labeled PKH67 interacted marrow stromal cells. The gain- loss-function method applied determine internalization secretion cells-derived EVs under intervention or NORAD. Results a high particularly metastasis. knockdown resulted EVs, suppressed It indicated that miR-541-3p, upregulation PKM2. Forced PKM2 promoted transfer PKH67-labeled Conclusions might serve as ceRNA miR-541-3p promote expression, thereby enhancing development promoting cells, providing insight into novel treatment for disorder.