Temporal phenotyping of circulating microparticles after trauma: a prospective cohort study

• DOI: 10.1186/s13049-018-0499-9
• Publication Date: 2018-04-26T22:55:59Z
• Authors: Matthias Fröhlich; Nadine Schäfer; Michael Caspers; Julia K. Böhm; Ewa K. Stürmer; Bertil Bouillon; Marc Maegele
• Keywords: Polytrauma

After severe polytrauma the dynamic process of coagulation may deteriorate towards a trauma-induced coagulopathy (TIC) promoting dramatic increase in morbidity and mortality. Recent evidence suggests that microparticles (MPs) play pivotal role at interface between cellular plasmatic systems. However, impact MPs on functional has not been clarified yet setting traumatic injuries. We assessed temporal patterns circulating MP concentrations including their origin context clinical presentation global assays. Blood samples from 22 consecutive patients (ISS ≥16) 2015 were collected hospital admission, after 24 72 h compared to those healthy individuals minor injured with isolated extremity fractures. Flow cytometry (BD Accuri C6; Heidelberg/Germany) was used determine using cell-specific markers (platelet derived (PDMP): CD42b+, CD61+, CD62p+; endothelial cell (EDMP): CD144+, CD62e+, CD144+/62e+). Results correlated data results viscoelastic testing (ROTEM). Twenty two (17 males, agemedian 60 yrs) median ISS 26.5 (IQR 14.5) assessed. PDMP EDMP increased significantly as patients. injury severity (CD144+: ρsp = 0.79, p < 0.001; CD42b+: 0.61, 0.001). displayed negative correlation aPTT (CD144/62e+, − 0.55, 0.05), INR 0.05) ROTEM-INTEM CT 0.68, reflecting dynamics clot formation an overall procoagulative effect. Additionally, showed association FIBTEM values (10 min amplitude, maximum firmness) indicating fibrinolytic potential. In small cohort, analysing most severly patients, levels altered parameters could be demonstrated. these findings are based analysis, which do enable causel evidence. Therefore, further in-vitro studies needed underlying pathomechanisms. conclusion, this study demonstrate following correlating severity. Although observed, associated improved suggesting essential for regulating blood trauma.