Single-cell transcriptome profiling of an adult human cell atlas of 15 major organs

• DOI: 10.1186/s13059-020-02210-0
• Publication Date: 2020-12-07T08:03:14Z
• Authors: Shuai He; Lin-He Wang; Yang Liu; Yi-Qi Li; Hai-Tian Chen; Jing-Hong Xu; Wan Peng; Guo-Wang Lin; Pan-Pan Wei; Bo Li; Xiaojun Xia; Dan Wang; Jin-Xin Bei; Xiaoshun He; Zhiyong Guo
• Keywords: Cell type; Single-Cell Analysis

Abstract Background As core units of organ tissues, cells various types play their harmonious rhythms to maintain the homeostasis human body. It is essential identify characteristics in organs and regulatory networks for understanding biological mechanisms related health disease. However, a systematic comprehensive single-cell transcriptional profile across multiple normal adult missing. Results We perform transcriptomes 84,363 derived from 15 tissue one donor generate an cell atlas. The atlas depicts 252 subtypes cells, including major such as T, B, myeloid, epithelial, stromal well novel COCH + fibroblasts FibSmo each which distinguished by marker genes profiles. These collectively contribute heterogeneity organs. Moreover, T B receptor repertoire comparisons trajectory analyses reveal direct clonal sharing with developmental states among different tissues. Furthermore, markers, transcription factors, ligand-receptor pairs are identified potential functional regulations maintaining Conclusions reveals inter- intra-organ provides useful resource uncovering key events during development diseases context