Lignocellulose conversion for biofuel: a new pretreatment greatly improves downstream biocatalytic hydrolysis of various lignocellulosic materials

• DOI: 10.1186/s13068-015-0419-4
• Publication Date: 2015-12-24T01:05:08Z
• Authors: Seung Gon Wi; Eun Jin Cho; Dae-Seok Lee; Soo Jung Lee; Young Ju Lee; Hyeun-Jong Bae
• Keywords: Lignocellulosic Biomass; Enzymatic Hydrolysis

Lignocellulosic biomass is an attractive renewable resource for future liquid transport fuel. Efficient and cost-effective production of bioethanol from lignocellulosic depends on the development a suitable pretreatment system. The aim this study to investigate new method that highly efficient effective downstream biocatalytic hydrolysis various materials, which can accelerate commercialization.The optimal conditions hydrogen peroxide-acetic acid (HPAC) were 80 °C, 2 h, equal volume mixture H2O2 CH3COOH. Compared organo-solvent under same conditions, HPAC was more at increasing enzymatic digestibility. After treatment, composition recovered solid 74.0 % cellulose, 20.0 hemicelluloses, 0.9 lignin. Notably, 97.2 lignin removed with pretreatment. Fermentation hydrolyzates by S. cerevisiae resulted in 412 mL ethanol kg(-1) after 24 equivalent 85.0 maximum theoretical yield (based amount glucose raw material).The newly developed removing cell walls, resulting enhanced accessibility substrate cellulose hydrolysis. This produced less amounts fermentative inhibitory compounds. In addition, enables year-round operations, maximizing utilization plant sources.