Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production

• DOI: 10.1186/s13068-020-01708-w
• Publication Date: 2020-04-15T14:02:41Z
• Authors: Eleni Stylianou; Chrysanthi Pateraki; Dimitrios Ladakis; María Cruz-Fernández; Marcos Latorre-Sá...; Caterina Coll; Apostolis Koutinas
• Keywords: 0106 biological sciences; 2. Zero hunger; Techno-economic evaluation; Research; Organic fraction of municipal solid waste; Continuous fermentation; Fuel; 01 natural sciences; 6. Clean water; 12. Responsible consumption; Succinic acid; TP315-360; 13. Climate action; 11. Sustainability; Actinobacillus succinogenes; Bioprocess; TP248.13-248.65; Biotechnology; 0105 earth and related environmental sciences

Abstract Background Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implementation.

Results Succinic acid production has been evaluated using hydrolysates from the organic fraction of municipal solid waste (OFMSW) collected from MSW treatment plants. A tailor-made enzymatic cocktail was used for OFMSW hydrolysate production containing up to 107.3 g/L carbon sources and up to 638.7 mg/L free amino nitrogen. The bacterial strains Actinobacillus succinogenes and Basfia succiniciproducens were evaluated for succinic acid production with the latter strain being less efficient due to high lactic acid production. Batch A. succinogenes cultures supplemented with 5 g/L yeast extract and 5 g/L MgCO3 reached 29.4 g/L succinic acid with productivity of 0.89 g/L/h and yield of 0.56 g/g. Continuous cultures at dilution rate of 0.06 h−1 reached 21.2 g/L succinic acid with yield of 0.47 g/g and productivity of 1.27 g/L/h. Downstream separation and purification of succinic acid was achieved by centrifugation, treatment with activated carbon, acidification with cation exchange resins, evaporation and drying, reaching more than 99% purity. Preliminary techno-economic evaluation has been employed to evaluate the profitability potential of bio-based succinic acid production.

Conclusions The use of OFMSW hydrolysate in continuous cultures could lead to a minimum selling price of 2.5 $/kg at annual production capacity of 40,000 t succinic acid and OFMSW hydrolysate production cost of 25 $/t sugars.