Tissue-specific regulation of Igf2r/Airn imprinting during gastrulation

• DOI: 10.1186/s13072-015-0003-y
• Publication Date: 2015-03-13T10:14:05Z
• Authors: Chelsea Marcho; Ariana Bevilacqua; Kimberly D Tremblay; Jesse Mager
• Keywords: Genomic Imprinting; Imprinting (psychology); Reprogramming

Appropriate epigenetic regulation of gene expression during lineage allocation and tissue differentiation is required for normal development. One example genomic imprinting, which defined as parent-of-origin mono-allelic expression. Imprinting established largely due to differences arriving in the zygote from sperm egg haploid genomes. In mouse, there are approximately 150 known imprinted genes, many occur clusters that regulated together. cluster includes maternally expressed Igf2r, Slc22a2, Slc22a3 genes paternally long non-coding RNA (lncRNA) Airn. Although it Igf2r Airn reciprocally imprinted, timing accompanying changes have not been well characterized vivo. Here we show lineage- temporal-specific DNA methylation histone modifications at Igf2r/Airn locus correlating with differential establishment gastrulation. Our results both alleles E6.5 epiblast. After gastrulation commences, becomes embryonic lncRNA paternal allele restricted maternal We document differentially enriched allele-specific extraembryonic tissues. also first time spreading concurrent Igf2r. Importantly, does change even though DMR2 occur, suggesting distinct mechanisms play lineages. These indicate similar preimplantation, represents a window dynamic lineage-specific