Co-regulation of transcription by BRG1 and BRM, two mutually exclusive SWI/SNF ATPase subunits

• DOI: 10.1186/s13072-017-0167-8
• Publication Date: 2017-12-22T06:20:41Z
• Authors: Jesse R. Raab; John S. Runge; Camarie C. Spear; Terry Magnuson
• Keywords: SWI/SNF; ARID1A; SMARCA4; Transcription

SWI/SNF is a large heterogeneous multi-subunit chromatin remodeling complex. It consists of multiple sets mutually exclusive components. Understanding how loss one sibling pair affects the occupancy and function remaining complex needed to understand mutations in particular subunit might affect tumor formation. Recently, we showed that members ARID family subunits (ARID1A, ARID1B ARID2) had transcriptional relationships including both antagonism cooperativity. However, it remains unknown catalytic subunit(s) binding genome-wide remainder changes output. We addressed this gap by depleting BRG1 BRM, two ATPase SWI/SNF, characterizing related transcription induced subunits. show depletion frequently leads subunit. This could cause either positive or negative gene expression. At subset sites, retained gained. Additionally, BRM have cooperative antagonistic interactions with respect transcription. Importantly, at genes where antagonize another observe nearly complete rescue expression combined BRG/BRM double knockdown. series experiments demonstrate complexes functional highlight importance considering role following single