Epigenome-wide association study of metabolic syndrome in African-American adults

• DOI: 10.1186/s13148-018-0483-2
• Publication Date: 2018-04-19T15:22:07Z
• Authors: Tomi Akinyemiju; Anh N. Do; Amit Patki; Stella Aslibekyan; Degui Zhi; Bertha Hidalgo; Hemant K. Tiwari; Devin Absher; Xin Geng; Donna K. Arnett; Marguerite R. Irvin
• Keywords: CpG site; Genome-wide Association Study; Epigenomics; Human genetics; Epigenome

The high prevalence of obesity among US adults has resulted in significant increases associated metabolic disorders such as diabetes, dyslipidemia, and blood pressure. Together, these constitute syndrome, a clinically defined condition highly prevalent African-Americans. Identifying epigenetic alterations with syndrome may provide additional information regarding etiology beyond current evidence from genome-wide association studies. Data on DNA methylation was assessed 614 African-Americans the Hypertension Genetic Epidemiology Network (HyperGEN) study. Metabolic using joint harmonized criteria, Illumina HumanMethylation450K Bead Chip assay extracted buffy coat. Linear mixed effects regression models were used to examine between CpG at > 450,000 sites adjusted for study covariates. Replication separate sample 69 African-Americans, well meta-analysis combining both cohorts, conducted. Two differentially methylated IGF2BP1 gene chromosome 17 (cg06638433; p value = 3.10 × 10− 7) ABCG1 21 (cg06500161; 2.60 8) identified. Results remained statistically replication dataset meta-analysis. consistently increased discovery datasets, that encodes protein ATP-binding cassette transporter family is involved intra- extra-cellular signaling lipid transport.