BM-MSC-derived exosomes alleviate radiation-induced bone loss by restoring the function of recipient BM-MSCs and activating Wnt/β-catenin signaling

• DOI: 10.1186/s13287-018-1121-9
• Publication Date: 2019-01-15T08:02:52Z
• Authors: Rui Zuo; Minghan Liu; Yanqiu Wang; Jie Li; Wenkai Wang; Junlong Wu; Chao Sun; Bin Li; Ziwen Wang; Weiren Lan; Chao Zhang; Chunmeng Shi; Yue Zhou
• Keywords: Exosome

Radiotherapy to cancer patients is inevitably accompanied by normal tissue injury, and the bone one of most commonly damaged tissues. Damage marrow mesenchymal stem cells (BM-MSCs) induced radiation thought be a major cause radiation-induced loss. Exosomes exhibit great therapeutic potential in treatment osteoporosis, but whether exosomes are involved loss has not been thoroughly elucidated date. The main purpose this study investigate role derived from BM-MSCs restoring recipient BM-MSC function alleviating BM-MSC-derived were intravenously injected rats immediately after irradiation. After 28 days, left tibiae harvested for micro-CT histomorphometric analysis. effects on antioxidant capacity, DNA damage repair, proliferation, cell senescence determined. Osteogenic adipogenic differentiation assays used detect BM-MSCs, related genes measured qRT-PCR Western blot β-Catenin expression was detected at histological cytological levels. can attenuate rat model that similar transplantation. Exosome-treated reduced oxidative stress, accelerated proliferation inhibition senescence-associate protein compared with exclusively received Following irradiation, promote β-catenin restore balance between osteogenic differentiation. Our findings indicate take BM-MSCs. Therefore, may represent promising cell-free approach