Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro

• DOI: 10.1186/s13287-020-01671-1
• Publication Date: 2020-05-24T08:02:32Z
• Authors: Behnaz Ashtari; Azar Shams; Narges Esmaeilzadeh; Sara Tanbakooei; Morteza Koruji; Mojtaba Johari Mo...; Javad Mohajer Ansari; Adel Johari Moghadam; Ronak Shabani
• Keywords: Stem cell marker

Abstract Background Some children who have survived cancer will be azoospermic in the future. Performing isolation and purification procedures for spermatogonial stem cells (SSC) is very critical. In this regard, performing process of decontamination cancerous initial step. The major objective present study to separate malignant EL4 cell line mice vitro. Methods sixty neonatal were isolated, procedure co-culturing was carried out by which classified into 2 groups: (1) control group (co-culture a growth medium) (2) co-cultured separated using microfluidic device. percentage assessed flow cytometry technique common laboratory immunocytochemistry finally confirmed through reverse transcription-polymerase chain reaction (RT-PCR). Results actual SSC after collected at two outlets: outputs smaller outlet 0.12% 42.14% EL4, while larger outlet, 80.38% 0.32% EL4; group, percentages 21.44% 23.28% (based on t test ( p ≤ 0.05)). Conclusions demonstrates that use device effective separating from cells.