Bone marrow mesenchymal stem cell-derived exosomes promote rotator cuff tendon-bone healing by promoting angiogenesis and regulating M1 macrophages in rats

• DOI: 10.1186/s13287-020-02005-x
• Publication Date: 2020-11-25T11:02:48Z
• Authors: Yao Huang; Bing He; Lei Wang; Bin Yuan; Hao Shu; Fucheng Zhang; Luning Sun
• Keywords: Rats, Sprague-Dawley; Tendons; Rotator Cuff; Research; Macrophages; Animals; Neovascularization, Physiologic; Mesenchymal Stem Cells; Exosomes; Rats

Abstract Background Rotator cuff tears (RCTs) often require reconstructive surgery. Tendon-bone healing is critical for the outcome of rotator reconstruction, but process tendon-bone complex and difficult. Mesenchymal stem cells (MSCs) are considered to be an effective method promote healing. MSCs have strong paracrine, anti-inflammatory, immunoregulatory, angiogenic potential. Recent studies shown that achieve many regulatory functions through exosomes. The purpose this study was explore role bone marrow mesenchymal cell-derived exosomes (BMSC-Exos) in Methods Our found BMSC-Exos proliferation, migration, tube formation human umbilical vein endothelial (HUVECs). mechanism by which may regulation signaling pathway. In addition, can inhibit polarization M1 macrophages secretion proinflammatory factors macrophages. After reconstruction rats, were injected into tail analyze their effect on interface Results It confirmed increased breaking load stiffness after induced angiogenesis around endpoint, promoted growth interface. Conclusion rats promoting inhibiting inflammation.