Wharton’s jelly mesenchymal stem cells embedded in PF-127 hydrogel plus sodium ascorbyl phosphate combination promote diabetic wound healing in type 2 diabetic rat

• DOI: 10.1186/s13287-021-02626-w
• Publication Date: 2021-10-30T16:02:38Z
• Authors: Yiren Jiao; Xiaolin Chen; Yongxia Niu; Sunxing Huang; Jingwen Wang; Mingxun Luo; Guang Shi; Junjiu Huang
• Keywords: Wharton's jelly

Abstract Background Diabetic cutaneous ulcers (DCU) are a complication of diabetes with diabetic foot being the most common, and wounds difficult to heal, increasing risk bacterial infection. Cell-based therapy utilizing mesenchymal stem cells (MSCs) is currently investigated as therapeutic avenue for both chronic severe burns. Wharton’s jelly cell (WJMSC) PF-127 hydrogel sodium ascorbyl phosphate (SAP) improved skin wound healing in mice. Whether this combination strategy helpful remains be explored. Methods Firstly, WJMSCs embedded SAP were transplanted onto excisional bed type 2 Sprague Dawley (SD) rats. Two weeks after transplantation, tissue was collected histological immunohistochemical analysis. Further, overexpressing-EGFP performed investigate engraftment ulcer. The apoptosis which encapsulated detected by TUNEL fluorescence assay RT-PCR vitro. And mitochondrial damage induced oxidative stress assessed MitoTracker CMH2DCFDA assay. Results In rat model, plus SAP-encapsulated transplantation promoted healing, indicating improving dermis regeneration collagen deposition. less pro-inflammatory M1 macrophages, more anti-inflammatory M2 tissue-healing neovascularization observed + group compared other groups. supplementation alleviated ratio vitro survival vivo. Conclusion facilitates WJMSCs-mediated through promoting survival, macrophage transformation, angiogenesis. Our findings may potentially provide patients