The non-structural protein μNS of piscine orthoreovirus (PRV) forms viral factory-like structures

• DOI: 10.1186/s13567-015-0302-0
• Publication Date: 2016-01-06T03:35:09Z
• Authors: Hanne Merethe Haa...; Ingvild B. Nyman; Turhan Markussen; Øystein Wessel; Maria Krudtaa Dahle; Espen Rimstad
• Keywords: Viral protein

Piscine orthoreovirus (PRV) is associated with heart- and skeletal muscle inflammation in farmed Atlantic salmon. The virus ubiquitous found both wild salmonid fish. It belongs to the family Reoviridae, closely related genus Orthoreovirus. PRV genome comprises ten double-stranded RNA segments encoding at least eight structural two non-structural proteins. Erythrocytes are major target cells for PRV. Infected erythrocytes contain globular inclusions resembling viral factories; putative site of replication. For mammalian reovirus (MRV), protein μNS primary organizer factory formation. analogous was focus present study. subcellular location its co-localization σNS, µ2 λ1 proteins investigated. We demonstrated that forms dense cytoplasmic transfected fish cells, factories MRV. In co-transfection experiments μNS, μ2 were recruited structures. ability recruit other into indicates it main involved formation pivotal early steps assembly.