A Mycobacterium avium subsp. paratuberculosis relA deletion mutant and a 35 kDa major membrane protein elicit development of cytotoxic T lymphocytes with ability to kill intracellular bacteria

• DOI: 10.1186/s13567-018-0549-3
• Publication Date: 2018-06-25T23:29:04Z
• Authors: Gaber S. Abdellrazeq; Mahmoud M. Elnaggar; John P. Bannantine; Kun T. Park; Cleverson D. Souza; Brian Backer; Victoria Hulubei; Lindsay M. Fry; Samy A. Khaliel; Helmy A. Torky; David A. Schneider; William C. Davis
• Keywords: CTL*; Granzyme

Efforts to develop live attenuated vaccines against Mycobacterium avium subspecies paratuberculosis (Map), using indirect methods screen Map deletion mutants for potential efficacy, have not been successful. A reduction in the capacity survive macrophages has predicted ability of vivo. Previous studies screening three cattle and goats revealed one mutant, with a relA (ΔMap/relA), could establish persistent infection. Further studies, antigen presenting cells (APC), blood dendritic monocyte derived DC, pulsed ΔMap/relA or 35 kDa membrane protein (MMP) component response was directed towards MMP. As reported herein, we developed bacterium viability assay cell culture assays analysis evaluation cytotoxic T generated Analysis effector activity responding reason infection that vaccination elicited development CD8 (CTL) kill intracellular bacteria. We demonstrated same CTL be two rounds antigenic stimulation APC MMP ex Cytotoxicity mediated through perforin granzyme B pathway. Finally, cognate recognition peptides presented context MHC I II molecules CD4 is required CTL.