Reproduction of contagious caprine pleuropneumonia reveals the ability of convalescent sera to reduce hydrogen peroxide production in vitro

• DOI: 10.1186/s13567-019-0628-0
• Publication Date: 2019-02-08T14:13:19Z
• Authors: Anne Liljander; Flavio Sacchini; Michael H. Stoffel; Elise Schieck; Nadine Stokar-Reg...; Fabien Labroussaa; Martin Heller; Jeremy Salt; Joachim Frey; Laurent Falquet; Danny Goovaerts; Joerg Jores
• Keywords: Mycoplasma mycoides

Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. capripneumoniae is a severe disease widespread in Africa and Asia. Limited knowledge available on the pathogenesis of this organism, mainly due to lack robust vivo challenge model means do site-directed mutagenesis. This work describes establishment novel for CCPP that resulted 100% morbidity using combination repeated intranasal spray infection followed single transtracheal employing recent Kenyan outbreak strain ILRI181. Diseased animals displayed CCPP-related pathology bacteria could subsequently be isolated from pleural exudates lung tissues concentrations up 109 per mL as well trachea immunohistochemistry. Reannotation genome sequence ILRI181 F38T revealed existence genes encoding complete glycerol uptake metabolic pathways involved hydrogen peroxide (H2O2) production phylogenetically related pathogen M. mycoides mycoides. Furthermore, expression L-α-glycerophosphate oxidase (GlpO) was confirmed. In addition, function metabolism verified measurement H2O2 medium containing physiological serum glycerol. Peroxide inhibited with convalescent animals. These results will pave way better understanding host–pathogen interactions during subsequent vaccine development.