Newcastle disease virus RNA-induced IL-1β expression via the NLRP3/caspase-1 inflammasome

• DOI: 10.1186/s13567-020-00774-0
• Publication Date: 2020-04-15T10:03:05Z
• Authors: Pei Gao; Libin Chen; Lei Fan; Jinlian Ren; Haoyun Du; Minhua Sun; Yaling Li; Peng Xie; Qiuyan Lin; Ming Liao; Chenggang Xu; Zhangyong Ning; Chan Ding; Bin Xiang; Tao Ren
• Keywords: Newcastle Disease; Pathogenesis

Abstract Newcastle disease virus (NDV) infection causes severe inflammation and is a highly contagious in poultry. Virulent NDV strains (GM) induce large quantities of interleukin-1β (IL-1β), which the central mediator inflammatory reaction. Excessive expression IL-1β exacerbates damage. Therefore, exploring mechanisms underlying NDV-induced can aid further understanding pathogenesis disease. Here, we showed that anti-IL-1β neutralizing antibody treatment decreased body temperature mortality following with virulent NDV. We explored primary molecules involved from perspective both host virus. This study overexpression NLRP3 resulted increased expression, whereas inhibition or caspase-1 caused significant reduction indicating NLRP3/caspase-1 axis expression. Moreover, ultraviolet-inactivated GM (chicken/Guangdong/GM/2014) failed to IL-1β. then collected GM-infected cell culture supernatant using ultracentrifugation, extracted viral RNA, stimulated cells RNA. The results revealed RNA alone was capable inducing RNA-induced Thus, our elucidated critical role while also demonstrating via antibodies damage associated infection; furthermore, induced NLRP3/caspase-1.