Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Ureaplasma urealyticum

• DOI: 10.1186/s13568-022-01357-2
• Publication Date: 2022-02-11T12:03:01Z
• Authors: Jie-Ni Shen; Jing-Yi Ye; Meng-Xiao Lao; Chu-Qiao Wang; Dong-Hong Wu; Xiao-Ying Chen; Li-Hong Lin; Wen-Yan Geng; Xu-Guang Guo
• Keywords: Ureaplasma urealyticum; Genital tract

Ureaplasma urealyticum (UU) is commonly present in human reproductive tract, which frequently leads to genital tract infection. Hence, there an urgent need develop a rapid detection method for UU. In our study, real-time fluorescence loop-mediated isothermal amplification (LAMP) assay was developed and evaluated the of Two primers were specifically designed based on highly conserved regions ureaseB genes. The reaction carried out 60 min constant temperature system using Bst DNA polymerase, process monitored by signal, while polymerase chain (PCR) performed simultaneously. LAMP system, positive result only obtained UU among 9 bacterial strains, with sensitivity 42 pg/μL (4.2 × 105 CFU/mL), all 16 clinical samples could be detected. conclusion, simple, sensitive, specific effective compared conventional PCR, shows great promise