Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs

• DOI: 10.1186/s40104-017-0216-y
• Publication Date: 2018-01-15T12:10:56Z
• Authors: Jiawei Wang; Chunfu Qin; Ting He; Kai Qiu; Wenjuan Sun; Xin Zhang; Ning Jiao; Weiyun Zhu; Jingdong Yin
• Keywords: Hindgut; Short-chain fatty acid; Faecalibacterium prausnitzii

Pork produced by outdoor-reared pigs raised mostly on alfalfa pastures attracts increasing population of consumer from most the world. In China, were with alfalfa-containing diets to seek for good quality pork. However, influence dietary involving high level insoluble fiber (IDF) pig intestinal luminal microbiota composition remains unclear. The objective this study was investigate effects and short chain fatty acids (SCFA) production, gene expressions involved in SCFA sensing, transporting absorbing caecal mucosa. Twenty-four growing randomly allotted four containing 0%, 5%, 10% 15% meal a 28-d experiment. Ingestion meal-contained significantly increased ratio body weight gain feed consumption. Illumina MiSeq sequencing V3 region 16S rRNA genes showed that diet decreased relative abundance genera Turicibacter, Acidiphilium, Paracoccus, Propionibacterium, Corynebacterium, Pseudomonas, Acinetobacter, Staphylococcus, Lachnospira, Marvinbryantia, Desulfovibrio digesta. Butyrate concentration hindgut supplementation diets. mRNA FFAR3, SMCT1, MCT1, PYY, GCG mucosa fed meal. Our results suggested has exerted significant impacts composition, butyrate upregulated expression host mucosal sensing absorption as well regulation satiety.