MiR-146a-5p targeting SMAD4 and TRAF6 inhibits adipogenensis through TGF-β and AKT/mTORC1 signal pathways in porcine intramuscular preadipocytes

• DOI: 10.1186/s40104-020-00525-3
• Publication Date: 2021-02-03T00:06:45Z
• Authors: Que Zhang; Rui Cai; Guorong Tang; Wanrong Zhang; Weijun Pang
• Keywords: 2. Zero hunger; 0303 health sciences; Adipogenesis; AKT/mTORC1 signal pathway; Veterinary medicine; Research; MiR-146a-5p; SMAD4; SF1-1100; Animal culture; 03 medical and health sciences; SF600-1100; TGF-β signal pathway; Porcine intramuscular fat

Abstract Background Intramuscular fat (IMF) content is a vital parameter for assessing pork quality. Increasing evidence has shown that microRNAs (miRNAs) play an important role in regulating porcine IMF deposition. Here, novel miRNA implicated adipogenesis was found, and its effect regulatory mechanism were further explored with respect to intramuscular preadipocyte proliferation differentiation. Results By adipose tissue sequencing analysis, we found miR-146a-5p potential regulator of adipogenesis. Further studies showed mimics inhibited differentiation, while the inhibitor promoted cell adipogenic Mechanistically, suppressed by directly targeting SMAD family member 4 ( SMAD4 ) attenuate TGF-β signaling. Moreover, differentiation preadipocytes TNF receptor-associated factor 6 TRAF6 weaken AKT/mTORC1 signaling downstream pathway. Conclusions MiR-146a-5p targets inhibit attenuating signaling, respectively. These findings provide biomarker promote