Feeding a Saccharomyces cerevisiae fermentation product improves udder health and immune response to a Streptococcus uberis mastitis challenge in mid-lactation dairy cows

• DOI: 10.1186/s40104-021-00560-8
• Publication Date: 2021-04-08T02:03:48Z
• Authors: M. Vailati-Riboni; D. N. Coleman; V. Lopreiato; A. Alharthi; R. E. Bucktrout; E. Abdel-Hamied; I. Martinez-Cortes; Y. Liang; E. Trevisi; I. Yoon; J. J. Loor
• Keywords: Udder; Somatic Cell Count; Streptococcus uberis

Abstract Background We aimed to characterize the protective effects and molecular mechanisms of action a Saccharomyces cerevisiae fermentation product (NTK) in response mastitis challenge. Eighteen mid-lactation multiparous Holstein cows ( n = 9/group) were fed control diet (CON) or CON supplemented with 19 g/d NTK for 45 d (phase 1, P1) then infected right rear quarter 2500 CFU Streptococcus uberis 2, P2). After 36-h, mammary gland liver biopsies collected antibiotic treatment started until end P2 (9 post challenge). Cows followed day 75 3, P3). Milk yield (MY) dry matter intake (DMI) recorded daily. samples somatic cell score collected, rectal udder temperature, heart respiration rate during challenge period (P2) together blood metabolite immune function analyses. Data analyzed by phase using PROC MIXED procedure SAS. Biopsies used transcriptomic analysis via RNA-sequencing, pathway analysis. Results DMI MY not affected P1, but an interaction time was indicating better recovery from compared CON. reduced score, temperature Transcriptome data supported these findings, as supplementation upregulated genes related antibacterial (e.g., CATHL4 , NOS2 ), epithelial tissue protection (e.g. IL17C anti-inflammatory activity ATF3 BAG3 IER3 G-CSF GRO1 ZFAND2A ). Pathway indicated upregulation tumor necrosis factor α, heat shock protein response, p21 pathways cows. Other detoxification cytoprotection functions along tight junction also NTK-fed Conclusions Overall, results highlighted networks involved effect prophylactic on health subclinical mastitic event.