Melatonin improves the first cleavage of parthenogenetic embryos from vitrified–warmed mouse oocytes potentially by promoting cell cycle progression

• DOI: 10.1186/s40104-021-00605-y
• Publication Date: 2021-07-16T00:02:54Z
• Authors: Bo Pan; Izhar Hyder Qazi; Shichao Guo; Jingyu Yang; Jianpeng Qin; Tianyi Lv; Shengqin Zang; Yan Zhang; Changjun Zeng; Qingyong Meng; Hongbing Han; Guangbin Zhou
• Keywords: Cleavage (geology); Pronucleus

Abstract Background This study investigated the effect of melatonin (MT) on cell cycle (G1/S/G2/M) parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II (MII) oocytes and elucidated potential mechanism MT action in first cleavage embryos. Results After vitrification warming, were parthenogenetically activated (PA) vitro cultured (IVC). Then spindle morphology chromosome segregation oocytes, maternal mRNA levels genes including Miss, Doc1r, Setd2 Ythdf2 pronuclear formation, S phase duration zygotes, mitochondrial function at G1 phase, reactive oxygen species (ROS) level DNA damage G2 early apoptosis 2-cell embryos, blastocyst formation rates evaluated. The results indicated that vitrification/warming procedures led to following perturbations 1) abnormalities misalignment, alteration mRNAs delay pronucleus 2) decreased membrane (MMP) lower adenosine triphosphate (ATP) levels, increased ROS production damage, G1/S S/G2 transition delay, delayed cleavage, 3) formation. Our further revealed such negative impacts oocyte cryopreservation could be alleviated by supplementation recovery, PA IVC media with 10 − 9 mol/L before embryos moved into stage development. Conclusions might promote progression via regulation MMP, ATP, potentially increasing vitrified–warmed their subsequent