UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1

• DOI: 10.1186/s40104-024-01043-2
• Publication Date: 2024-06-11T00:02:07Z
• Authors: Shengjie Shi; Huan Yuan; Lutong Zhang; Lei Gao; Lili Zhao; Xiangfang Zeng; Shiyan Qiao; Guiyan Chu; Chuanjiang Cai
• Keywords: 0301 basic medicine; Granulosa cells; 03 medical and health sciences; Veterinary medicine; Research; Isovitexin; Proliferation; SF600-1100; CCNB1; UCHL1; SF1-1100; Animal culture

The proliferation of porcine ovarian granulosa cells (GCs) is essential to follicular development and the ubiquitin-proteasome system necessary for maintaining cell cycle homeostasis. Previous studies found that deubiquitinase ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) regulates female reproduction, especially in development. However, mechanism by which UCHL1 GC remains unclear. overexpression promoted proliferation, knockdown had opposite effect. directly bound cyclin B1 (CCNB1), prolonging half-life CCNB1 inhibiting its degradation, thereby promoting proliferation. What's more, a flavonoid compound-isovitexin improved enzyme activity GCs. GCs stabilizing CCNB1, isovitexin enhanced UCHL1. These findings reveal role potential regulating provide insights into identifying molecular markers nutrients affect follicle