In silico analyses of metagenomes from human atherosclerotic plaque samples

• DOI: 10.1186/s40168-015-0100-y
• Publication Date: 2015-08-20T14:44:01Z
• Authors: Suparna Mitra; Daniela I. Drautz...; Morten Alhede; Myat T. Maw; Yang Liu; Rikky W. Purbojati; Zhei H. Yap; Kavita K. Kushwaha; Alexandra G. Gheo...; Thomas Bjarnsholt; Gorm M. Hansen; Henrik H. Sillesen; Hans P. Hougen; Peter R. Hansen; Liang Yang; Tim Tolker-Nielsen; Stephan C. Schuster; Michael Givskov
• Keywords: Medical microbiology

Through several observational and mechanistic studies, microbial infection is known to promote cardiovascular disease. Direct of the vessel wall, along with risk factors, hypothesized play a key role in atherogenesis by promoting an inflammatory response leading endothelial dysfunction generating proatherogenic prothrombotic environment ultimately clinical manifestations disease, e.g., acute myocardial infarction or stroke. There are many reports DNA isolation even few studies viable microbes isolated from human atherosclerotic vessels. However, high-resolution investigation infectious agents vessels that may contribute atherosclerosis very limited. In spite progress recent sequencing technologies, analyzing host-associated metagenomes remain challenge.To investigate microbiome diversity within tissue samples, we employed high-throughput metagenomic analysis on: (1) plaques obtained group patients who underwent endarterectomy due transient cerebral ischemia (2) Presumed stabile autopsy control all died causes not related Our data provides evidence suggest wide range plaques, intriguing new observation shows these microbiota displayed differences between symptomatic asymptomatic as judged taxonomic profiles two groups patients. Additionally, functional annotations reveal significant basic metabolic disease pathway signatures groups.We demonstrate feasibility novel techniques aimed at identification characterization genomes samples. suggests distinct might different roles during development plaques. These findings serve reference point for future this area research.