Mechanistic strategies of microbial communities regulating lignocellulose deconstruction in a UK salt marsh

• DOI: 10.1186/s40168-020-00964-0
• Publication Date: 2021-02-19T00:34:39Z
• Authors: Daniel R. Leadbeater; Nicola C. Oates; Joseph P. Bennett; Yi Li; Adam A. Dowle; Joe D. Taylor; Juliana Sanchez A...; Alexander T. Setc...; Anna M. Alessi; Thorunn Helgason; Simon J. McQueen-...; Neil C. Bruce
• Keywords: Salt marsh; Spartina alterniflora; Deltaproteobacteria

Abstract Background Salt marshes are major natural repositories of sequestered organic carbon with high burial rates matter, produced by highly productive native flora. Accumulated predominantly exists as lignocellulose which is metabolised communities functionally diverse microbes. However, the organisms that orchestrate this process and enzymatic mechanisms employed regulate accumulation, composition permanence stock not yet known. We applied meta-exo-proteome proteomics 16S rRNA gene profiling to study decomposition in situ within surface level sediments a established UK salt marsh. Results Our studies revealed community dominated Gammaproteobacteria , Bacteroidetes Deltaproteobacteria drive degradation identify 42 families lignocellulolytic bacteria most active secretors carbohydrate-active enzymes were observed be Prolixibacteracea Flavobacteriaceae Cellvibrionaceae Saccharospirillaceae Alteromonadaceae Vibrionaceae Cytophagaceae . These secreted lignocellulose-active glycoside hydrolase (GH) family GH3, GH5, GH6, GH9, GH10, GH11, GH13 GH43 associated degrading Spartina biomass. While fungi present, we did detect contribution from contributors terrestrial deconstruction. Oxidative such laccases, peroxidases lytic polysaccharide monooxygenases important for environment present but abundant, while notable abundance putative esterases (such carbohydrate esterase 1) decoupling lignin polysaccharides was observed. Conclusions Here, cohort previously undefined marsh describe they employ facilitate process. results increase understanding microbial molecular underpin sequestration provide insights into potential regulating enrichment polyphenolics sediments.