Effect of a multi-strain probiotic mixture consumption on anxiety and depression symptoms induced in adult mice by postnatal maternal separation

• DOI: 10.1186/s40168-024-01752-w
• Publication Date: 2024-02-19T04:12:41Z
• Authors: Francesca De Santa; Georgios Strimpakos; Nicole Marchetti; Giorgio Gargari; Alessio Torcinaro; Stefania Arioli; Diego Mora; Carla Petrella; Stefano Farioli-V...
• Keywords: Inflammation; Male; Mammals; 0301 basic medicine; Adult neurogenesis; Anxiety and depression; Gut-brain axis; Inflammation; Microbiota; Probiotic; Short-chain fatty acids; Depression; Microbiota; Research; Maternal Deprivation; Probiotics; QR100-130; Brain; Anxiety; Adult neurogenesis; Probiotic; Microbial ecology; Short-chain fatty acids; Mice; 03 medical and health sciences; Animals; Anxiety and depression; Gut-brain axis

Abstract Background Intestinal microbial composition not only affects the health of the gut but also influences centrally mediated systems involved in mood, through the “gut-brain” axis, a bidirectional communication between gut microbiota and the brain. In this context, the modulation of intestinal microbiota and its metabolites through the administration of probiotics seems to represent a very promising approach in the treatment of the central nervous system alterations. Early postnatal life is a critical period during which the brain undergoes profound and essential modulations in terms of maturation and plasticity. Maternal separation (MS), i.e., the disruption of the mother–pup interaction, represents a pivotal paradigm in the study of stress-related mood disorders, by inducing persistent changes in the immune system, inflammatory processes, and emotional behavior in adult mammals.

Results We conducted experiments to investigate whether sustained consumption of a multi-strain probiotic formulation by adult male mice could mitigate the effects of maternal separation. Our data demonstrated that the treatment with probiotics was able to totally reverse the anxiety- and depressive-like behavior; normalize the neuro-inflammatory state, by restoring the resting state of microglia; and finally induce a proneurogenic effect. Mice subjected to maternal separation showed changes in microbiota composition compared to the control group that resulted in permissive colonization by the administered multi-strain probiotic product. As a consequence, the probiotic treatment also significantly affected the production of SCFA and in particular the level of butyrate.

Conclusion Gut microbiota and its metabolites mediate the therapeutic action of the probiotic mix on MS-induced brain dysfunctions. Our findings extend the knowledge on the use of probiotics as a therapeutic tool in the presence of alterations of the emotional sphere that significantly impact on gut microbiota composition.