Dapagliflozin promotes white adipose tissue browning though regulating angiogenesis in high fat induced obese mice

• DOI: 10.1186/s40360-024-00747-5
• Publication Date: 2024-03-19T09:05:16Z
• Authors: Lin Xiang; Min Liu; Guangda Xiang; Ling Yue; Junxia Zhang; Xiaoli Xu; Jing Dong
• Keywords: Dapagliflozin; Browning; Lipogenesis; Steatosis

Abstract Browning of white adipose tissue (WAT) is become an appealing target for therapeutics in the treatment obesity and related metabolic diseases. Dapagliflozin widely used type 2 diabetes, it also found that drug exhibits regulate systemic metabolism such as obesity, insulin resistance hepatic steatosis. However, precise role dapagliflozin on WAT remodeling remains to be elucidated. The current study aimed explore browning high-fat diet (HFD)-induced obese mice. Male C57BL/6J mice ( n = 6 per group) were establish model by following feeding with HFD weeks. randomly treated or without experimental observation. volume fat fraction quantified, H&E, UCP-1 staining immunohistochemistry conducted investigate white-to-brown conversion angiogenesis respectively. Quantitative real-time polymerase chain reaction (qPCR) was employed mRNA expression levels genes WAT. Subsequently, 3T3-L1 cells effect preadipocytes differentiation vitro. Our results demonstrated could reduce body weight gain promote induced via regulating lipogenesis Furthermore, differentiation, up-regulate adipocytes In conclusion, can potentially improving