Can anti-bothropstoxin-I antibodies discriminate between Bothrops jararaca and Bothrops jararacussu venoms?

• DOI: 10.1186/s40409-017-0105-z
• Publication Date: 2017-03-11T03:34:18Z
• Authors: Ricardo Teixeira ...; Carlos Corrêa-Netto; Leonora Brazil-Más; Caio Raony Farina...; Irene Fernandes; Russolina Benedet...
• Keywords: Bothrops; Bothrops jararaca; Antivenom; Polyclonal antibodies; Ophidia

Snakes of the genus Bothrops, popularly known as pit vipers, are responsible for most cases snakebite in Brazil. Within this genus, Bothrops jararacussu and B. jararaca deserve special attention due to severity their bites inhabiting densely populated areas. Regarding treatment snakebites by jararacussu, questions have been raised about effectiveness specific bothropic antivenom neutralizing myotoxic effects; however, there no accurate data humans. Thus, development a differential diagnostic kit species would be great interest because it provides, healthcare professionals, tool that allow us determine whether accident was caused or other genus. It also make possible evaluate specificity provide epidemiological studies. First, we produced species-specific polyclonal antibody – potential biomarker venom against bothropstoxin-I (BthTx-I), which is found smaller quantities venoms from southern Polyclonal antibodies could separated into several immunoglobulins. Then, aiming develop system safe standardized immunoassay, monoclonal antibodies. Seven hybridomas were obtained. Five them two recognized southeastern population. The use made differentiate obtained Analyzing reactivity venoms, mAb Bt-3 more than others venom. These results show BthTx-I producing between venoms.