Mesenchymal stem cells in osteotomy repair after tibial tuberosity advancement in dogs with cranial cruciate ligament injury

• DOI: 10.1186/s40634-018-0130-z
• Publication Date: 2018-06-14T00:57:58Z
• Authors: Clarissa Rocha do...; Richard da Rocha ...; Patrícia Furtado ...; Andre Rodrigues d...; Kaique Nogueira; Carolina da Silva...; Eduardo Maurício ...
• Keywords: Posterior cruciate ligament

The cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used obtain rapid recovery of affected knee. objective study was evaluate viability use mesenchymal stem cells (MSC) implanted osteotomy site obtained by TTA nine dogs diagnosed with CCLR. MSC were isolated from adipose tissue and cultured for eight days, animals divided into two groups. Animals treated group (GT) received cell transport medium containing about 1.5 millions MSC, control (GC) only medium. performed a double-blind manner using radiographs acquired on days 15, 30, 60 120 after procedure. Evaluations density trabecular bone image analysis software. results subjected descriptive statistical analysis, followed normality test, Chi-square Mann-Whitney test Tukey’s multiple comparison p ≤ 0.05. After 30 procedure, GT presented an ossification mean 36.45% greater (p 0.033) than GC, there no differences other periods. Despite total within expected period, minimization estimated time application inflammatory factors should be considered reassessment therapeutic intervention time.