Enhancing transglutaminase production of Streptomyces mobaraensis by iterative mutagenesis breeding with atmospheric and room-temperature plasma (ARTP)

• DOI: 10.1186/s40643-017-0168-2
• Publication Date: 2017-08-12T07:50:48Z
• Authors: Ying Jiang; Yue-Peng Shang; Hao Li; Chao Zhang; Jiang Pan; Yun-Peng Bai; Chun-Xiu Li; Jian-He Xu
• Keywords: Strain (injury); Zymogen; Tissue transglutaminase

To improve the fermentation production of transglutaminase (TGase) from Streptomyces mobaraensis for applications in food industry, atmospheric and room-temperature plasma (ARTP) mutagenesis was applied to breed S. mutants with increased TGase production. After eight rounds iterative ARTP mutagenesis, four genetically stable mutants, Sm5-V1, Sm6-V13, Sm2-V10, Sm7-V12, were identified, which showed by 27, 24, 19%, respectively. The best mutant Sm5-V1 exhibited a maximum activity 5.85 U/mL during flask fermentation. Compared wild-type strain, transcription levels zymogen genes significantly as indicated quantitative real-time PCR, while gene nucleotide sequences did not change at all. It shown that overexpression contributes increase is potentially efficient tool microbial mutation breeding bring some significant changes required industrial applications.