Development of an economical fermentation platform for enhanced ansamitocin P-3 production in Actinosynnema pretiosum

• DOI: 10.1186/s40643-018-0235-3
• Publication Date: 2019-01-03T00:46:51Z
• Authors: Ting Liu; Lyailya Bessembayeva; Jun Chen; Liu-Jing Wei; Qiang Hua
• Keywords: Laboratory flask; Isobutanol

Ansamitocin P-3 (AP-3), produced by Actinosynnema pretiosum, has extraordinary antitumor activity and been used as toxic "warhead" in antibody-conjugated drugs. However, AP-3 production is limited its low yield thus the high cost of fermentation. In this study, we aimed to reduce fermentation costs utilizing low-cost substrates improve production, simultaneously. for ansamitocins were selected via single-factor experiments then concentrations these suitable carbon nitrogen sources optimized response surface method (RSM). Cane molasses, glycerol, cold-pressed soybean powder determined be most economical accumulation. The titer shaking flasks reached 111.9 mg/L using medium containing cane molasses (63.22 g/L), glycerol (22.91 (3.29 g/L). addition, appropriate supplementation with isobutanol, oil, vitamin B1 resulted a marked enhancement 141 mg/L. Meanwhile, was around 50% lower than those two other high-yielding media. Our findings provided practical economically competitive cultivation conditions value-added agent may extend applications ansamitocin P-3.