Diosmetin inhibits cell proliferation and promotes apoptosis through STAT3/c-Myc signaling pathway in human osteosarcoma cells

• DOI: 10.1186/s40659-021-00363-1
• Publication Date: 2021-12-18T11:14:26Z
• Authors: Rende Ning; Guang Chen; Run Fang; Yanhui Zhang; Wenjuan Zhao; Feng Qian
• Keywords: Survivin

Abstract Background Diosmetin is a bioflavonoid compound naturally abundant in citrus fruits. It found to perform variety of activities, while its antitumor property osteosarcoma, malignant tumor with unmet clinical treatment, remained unknown. Methods Colony formation assay, cell cycle analysis and apoptosis were conducted respectively observe the effect diosmetin on proliferation human osteosarcoma cells. Western blot immunoprecipitation used detect expression apoptotic molecules activation STAT3/c-Myc pathway Saos-2 U2SO Results significantly inhibited proliferation, induced arrest at G2/M phase promoted both Moreover, downregulated anti-apoptotic protein Bcl-xL upregulated levels pro-apoptotic proteins including cleaved Caspase-3, cleaved-PARP Bax. Furthermore, dose-dependently STAT3 phosphorylation, reduced downstream c-Myc impeded interaction between molecules. Conclusions These results suggest that exerts anti-osteosarcoma effects by suppressing inducing via inhibiting signaling pathway, which provide possibility for be chemotherapeutic candidate osteosarcoma.