DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development

• DOI: 10.1186/s40813-021-00235-w
• Publication Date: 2021-10-18T09:29:40Z
• Authors: Alma López; Miguel Betancourt; Yvonne Ducolomb; Juan José Rodríguez; Eduardo Casas; Edmundo Bonilla; Iván Bahena; Socorro Retana-Má...; Lizbeth Juárez-Rojas; Fahiel Casillas
• Keywords: Vitrification; Comet Assay; Cryoprotectant; In vitro maturation; Fertilisation

Abstract Background The evaluation of the DNA damage generated in cumulus cells after mature cumulus-oocyte complexes vitrification can be considered as an indicator oocyte quality since these play important roles developmental competence. Therefore, aim this study was to determine if matured exposure cryoprotectants (CPAs) or affects oocytes and viability, but also is cells, affecting fertilization embryo development. Results measured using alkaline comet assay expressed Comet Tail Length (CTL) Olive Moment (OTM). demonstrate that CPAs reduced (75.5 ± 3.69%, Toxicity; 66.7 4.57%, Vitrification) viability (32.7 5.85%, 7.7 2.21%, compared control (95.5 4.04%, oocytes; 89 4.24%, cells). Also, significantly higher OTM (39 17.41, 33.6 16.69, respectively) (7.4 4.22). In addition, development rates decreased (35.3 16.65%, 22.6 3.05%, (32.3 9.29%, 20 1%, respectively). It found granulose-intact were denuded groups. However, a decline blastocyst stage observed (1.66 0.57%) (2 1%) (22.3 2.51%). This could attributed reduction both cell types generation cells. Conclusion demonstrates rates. These findings will allow understand some mechanisms compromise their capacity, well search for new strategies increase by preserving integrity