Redirecting differentiation of mammary progenitor cells by 3D bioprinted sweat gland microenvironment

• DOI: 10.1186/s41038-019-0167-y
• Publication Date: 2019-09-22T23:02:56Z
• Authors: Rui Wang; Yihui Wang; Bin Yao; Tian Hu; Zhao Li; Yufan Liu; Xiaoli Cui; Liuhanghang Cheng; Wei Song; Sha Huang; Xiaobing Fu
• Keywords: Progenitor; 3D bioprinting

Mammary progenitor cells (MPCs) maintain their reproductive potency through life, and specific microenvironments exert a deterministic control over these cells. MPCs provides one kind of ideal tools for studying engineered microenvironmental influence because its accessibility continually undergoes postnatal developmental changes. The aim our study is to explore the critical role sweat gland (SG) microenvironment in reprogramming into functional SG cells.We have utilized three-dimensional (3D) composed gelatin-alginate hydrogels components from mouse extracellular matrix (SG-ECM) proteins reroute differentiation functions this microenvironment. were encapsulated artificial printed 3D cell-laden construct. expression markers at protein gene levels was detected after cultured 14 days.Compared with group, immunofluorescence assay demonstrated that bioprinted 3D-SG could significantly express marker SG, sodium/potassium channel ATP1a1, tend luminal epithelial cells, keratin-8. When Shh pathway inhibited, SG-associated under same induction environment reduced.Our evidence proved ability differentiated regenerate by vitro found be correlated changes differentiation. These results provide insights regeneration damaged cell fate.